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Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: SW480 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: SW480 whole cell lysate Lysates/proteins at 20 ug per lane.

MLK1 Antibody

CATALOG NUMBER: 63-287

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MLK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1070-1104 amino acids from the C-terminal region of human MLK1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
122 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAP3K9
Additional Names:
Mitogen-activated protein kinase kinase kinase 9, Mixed lineage kinase 1, MAP3K9, MLK1, PRKE1
Protein Accession Number:
P80192
Protein Gi Number:
116242625
Ncbi Gene Id Number:
4293
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MLK1 is a MLK(MAP3K) type protein kinase. The catalytic domain of mixed-lineage kinases (MLKs) kinases have amino acid sequence similarity to both the tyr-specific and the ser/thr-specific kinase classes. In addition to thee unusual nature of the kinase catalytic domains, MLK1 and MLK2 contain 2 leu/ile-zipper motifs and a basic sequence near their C-termini. MLK1 is a member of the neuronal apoptotic JNK/c-Jun pathway acting between Rac1/Cdc42 and MKK4 and -7 in death signaling. MLK1 expression has been documented in human epithelial tumor cell lines of colonic, breast and esophageal origin.
Background Reference 1:
Durkin, J.T., et al., Biochemistry 43(51):16348-16355 (2004).
Background Reference 2:
Gallo, K.A., et al., J. Biol. Chem. 269(21):15092-15100 (1994).
Background Reference 3:
Dorow, D.S., et al., Eur. J. Biochem. 213(2):701-710 (1993).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-287

List Size:
400 ul

List Price:

$495.00

Datasheet
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