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45-890 (4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
45-890 (3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
45-890 (4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

MLL2 Antibody

CATALOG NUMBER: 45-890

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: DSQNLAGEDKDSQ-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Using our routine western blotting protocol we would not expect to reliably detect proteins larger than 250kDa (calculated MW of approx. 593KDa according to NP_003473.3).Immunohistochemistry:In paraffin embedded Human Placenta shows nuclear stainng in the trophoblasts . Similar nuclear patches are observed in Human Liver. Recommended concentration: 4-8ug/ml.
Specificity:
The immunizing peptide was designed from NP_003473.1. Since then the sequence has changed in the databases and currently differs at two locations in NP_003473.3.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MLL2
Additional Names:
MLL2, myeloid/lymphoid or mixed-lineage leukemia 2, ALR, ALL1-related gene, AAD10, CAGL114, KMT2D, MLL4, TNRC21, ALL1-related, trinucleotide repeat containing 21, KMS, KABUK1, Lysine methyltransferase 2D
Protein Accession Number:
NP_003473.3
Protein Gi Number:
4505197
Ncbi Gene Id Number:
8085
Background Reference 1:
Prasad R, Zhadanov AB, Sedkov Y, Bullrich F, Druck T, Rallapalli R, Yano T, Alder H, Croce CM, Huebner K, Mazo A, Canaani E. Structure and expression pattern of human ALR, a novel gene with strong homology to ALL-1 involved in acute leukemia and to Drosophila trithorax. Oncogene. 1997 Jul 31;15(5):549-60.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-890

List Size:
0.1 mg

List Price:

$469.00

Out of stock

Datasheet
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