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Western Blot at 1:2000 dilution + Hela whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: Raji whole cell lysates Lane 2: Hela whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-MLLT1 Pab in A257 cell line lysate (35ug/lane).
Western Blot at 1:2000 dilution + Hela whole cell lysates Lysates/proteins at 20 ug per lane.

MLLT1 Antibody

CATALOG NUMBER: 62-162

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MLLT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 523-552 amino acids from the C-terminal region of human MLLT1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
62 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MLLT1
Additional Names:
Protein ENL, YEATS domain-containing protein 1, MLLT1, ENL, LTG19, YEATS1
Protein Accession Number:
Q03111
Protein Gi Number:
116241350
Ncbi Gene Id Number:
4298
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Chromosome band 11q23 is the site of translocations in myeloid and lymphoid acute leukemias, pediatric leukemias, and treatment-induced secondary acute myelogenous leukemia. The translocation breakpoints cluster in a restricted region of the HRX gene resulting in chimeric genes that encode an N-terminal portion of Hrx fused to various partner proteins. Myeloid/lymphoid or mixed-lineage leukemia translocated to 1 (MLLT1) is a nuclear protein with transcriptional transactivation properties that is fused to Hrx in t(11;19) leukemias. The minimal MLLT1 sequence required for transcription activation was narrowed to the C-terminal 90 amino acids.
Background Reference 1:
Nie, Z., et al., Mol. Cell. Biol. 23(8):2942-2952 (2003).
Background Reference 2:
Lavau, C., et al., Proc. Natl. Acad. Sci. U.S.A. 97(20):10984-10989 (2000).
Background Reference 3:
Thirman, M.J., et al., Proc. Natl. Acad. Sci. U.S.A. 91(25):12110-12114 (1994).
Background Reference 4:
Rubnitz, J.E., et al., Blood 84(6):1747-1752 (1994).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-162

List Size:
400 ul

List Price:

$495.00

Datasheet
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