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Western blot analysis of extracts of various cell lines, using MLN Antibody (22-179) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human stomach using MLN antibody (22-179) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MLN antibody (22-179) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using MLN antibody (22-179) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MLN antibody (22-179). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using MLN Antibody (22-179) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

MLN Antibody

CATALOG NUMBER: 22-179

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-115 of human MLN (NP_002409.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
MCF7
Positive Control 2:
SGC-7901
Positive Control 3:
HT-29
Positive Control 4:
Mouse stomach
Positive Control 5:
Mouse intestine
Predicted Molecular Weight:
Observed: 13kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MLN
Additional Names:
promotilin, prepromotilin
Ncbi Gene Id Number:
4295
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a small peptide hormone that is secreted by cells of the small intestine to regulate gastrointestinal contractions and motility. Proteolytic processing of the secreted protein produces the mature peptide and a byproduct referred to as motilin-associated peptide (MAP). Three transcript variants encoding different preproprotein isoforms but the same mature peptide have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-179

List Size:
100 uL

List Price:

$436.00

Datasheet
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