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Western blot analysis in A375 cell line lysates (35ug/lane).
MLPH Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MLPH Antibdy for immunohistochemistry.
Western blot analysis in A375 cell line lysates (35ug/lane).

MLPH Antibody

CATALOG NUMBER: 56-536

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MLPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 541-569 amino acids from the C-terminal region of human MLPH.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
66 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MLPH
Additional Names:
Melanophilin, Exophilin-3, Slp homolog lacking C2 domains a, SlaC2-a, Synaptotagmin-like protein 2a, MLPH, SLAC2A
Protein Accession Number:
Q9BV36
Protein Gi Number:
32129730
Ncbi Gene Id Number:
79083
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the exophilin subfamily ofRab effector proteins. The protein forms a ternary complex with thesmall Ras-related GTPase Rab27A in its GTP-bound form and the motorprotein myosin Va. A similar protein complex in mouse functions totether pigment-producing organelles called melanosomes to the actincytoskeleton in melanocytes, and is required for visiblepigmentation in the hair and skin. A mutation in this gene resultsin Griscelli syndrome type 3, which is characterized by asilver-gray hair color and abnormal pigment distribution in thehair shaft. Several alternatively spliced transcript variants ofthis gene have been described, but the full-length nature of someof these variants has not been determined.
Background Reference 1:
Park, J.W., et al. J. Med. Genet. 43(7):598-608(2006)
Background Reference 2:
Seabra, M.C., et al. Traffic 5(6):393-399(2004)
Background Reference 3:
Fukuda, M., et al. J. Cell. Sci. 117 (PT 4), 583-591 (2004) :
Background Reference 4:
Lukusa, T., et al. Genet. Couns. 15(3):293-301(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-536

List Size:
400 ul

List Price:

$495.00

Datasheet
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