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42-272 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-272 (3.8ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

MLX Antibody

CATALOG NUMBER: 42-272

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QIVKAHQDNPHEGED
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments in Human, Mouse and Rat Liver lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Placenta shows nuclear stsaining of trophoblasts. Recommended concentration, 2.5-3.8ug/ml.
Specificity:
This antibody is expected to recognize all three reported isoforms (NP_937848.1; NP_937847.1; NP_733752.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MLX
Additional Names:
MLX, MAX-like protein X, MAD7, MXD7, TCFL4, bHLHd13, BigMax protein, MAX-like bHLHZIP protein, transcription factor-like 4, transcription factor-like protein 4
Protein Accession Number:
NP_937848.1,NP_937847.1,NP_733752.1
Protein Gi Number:
38201614
Ncbi Gene Id Number:
6945
Background Reference 1:
Stoltzman CA, Peterson CW, Breen KT, Muoio DM, Billin AN, Ayer DE, Glucose sensing by MondoA:Mlx complexes: a role for hexokinases and direct regulation of thioredoxin-interacting protein expression. Proceedings of the National Academy of Sciences of the United States of America 2008 May 105 (19): 6912-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-272

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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