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Western blot analysis of MLX Antibody in MDA-MB231 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma with MLX Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H292 cells using MLX Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of MLX using rabbit polyclonal MLX Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLX gene.
Western blot analysis of MLX Antibody in MDA-MB231 cell line lysates (35ug/lane)

MLX Antibody

CATALOG NUMBER: 63-563

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This MLX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-151 amino acids from the Central region of human MLX.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MLX
Additional Names:
Max-like protein X, Class D basic helix-loop-helix protein 13, bHLHd13, Max-like bHLHZip protein, Protein BigMax, Transcription factor-like protein 4, MLX, BHLHD13, TCFL4
Protein Accession Number:
Q9UH92
Protein Gi Number:
20138856
Ncbi Gene Id Number:
6945
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MLX belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This protein may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4.
Background Reference 1:
Meroni,G., et.al., Oncogene 19 (29), 3266-3277 (2000)
Background Reference 2:
Billin,A.N., et.al., J. Biol. Chem. 274 (51), 36344-36350 (1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-563

List Size:
400 ul

List Price:

$495.00

Datasheet
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