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45-090 (0.03ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-090 (3.8ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-090 (0.03ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MMP-7 Antibody

CATALOG NUMBER: 45-090

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QKLYGKRSNSRKK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 28kDa band observed in Human Breast, Ovary and Placenta lysates (calculated MW of 29.7kDa according to NP_002414.1). Recommended concentration: 0.03-0.1ug/ml.Immunohistochemistry:In paraffin embedded Human Uterus shows basal staining of an endometrial gland. Recommended concentration, 3-6ug/ml.
Positive Control 1:
Cat. No. 1311 - Human Breast Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MMP7
Additional Names:
MMP7, matrix metalloproteinase 7 (matrilysin, uterine), HGNC:7174, MMP-7, MPSL1, PUMP-1, matrin, matrix metalloproteinase 7, uterine matrilysin, matrix metallopeptidase 7
Protein Accession Number:
NP_002414.1
Protein Gi Number:
4505219
Ncbi Gene Id Number:
4316
Background Reference 1:
Li YJ, Wei ZM, Meng YX, Ji XR. beta-catenin up-regulates the expression of cyclinD1, c-myc and MMP-7 in human pancreatic cancer: Relationships with carcinogenesis and metastasis. World J Gastroenterol. 2005 Apr 14;11(14):2117-23.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-090

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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