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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
MMP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of MMP1 Antibody with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).

MMP1 Antibody

CATALOG NUMBER: 56-091

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-347 amino acids from the Central region of human MMP1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MMP1
Additional Names:
Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1, 22 kDa interstitial collagenase, 27 kDa interstitial collagenase, MMP1, CLG
Protein Accession Number:
P03956
Protein Gi Number:
116852
Ncbi Gene Id Number:
4312
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Proteins of the matrix metalloproteinase (MMP) family areinvolved in the breakdown of extracellular matrix in normalphysiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in diseaseprocesses, such as arthritis and metastasis. Most MMP's aresecreted as inactive proproteins which are activated when cleavedby extracellular proteinases. This gene encodes a secreted enzymewhich breaks down the interstitial collagens, types I, II, and III.The gene is part of a cluster of MMP genes which localize tochromosome 11q22.3. Alternative splicing results in multipletranscript variants.
Background Reference 1:
Beshir, A.B., et al. Cancer Lett. 299(2):137-149(2010)
Background Reference 2:
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Background Reference 3:
Malik, N., et al. J. Neurooncol. (2010) In press :
Background Reference 4:
Skorupski, P., et al. Ginekol. Pol. 81(8):594-599(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-091

List Size:
400 ul

List Price:

$495.00

Datasheet
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