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Western blot analysis of hMMP10-R409 in mouse spleen tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of hMMP10-R409 in mouse spleen tissue lysates (35ug/lane)

MMP10 Antibody

CATALOG NUMBER: 62-167

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MMP10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 amino acids from the C-terminal region of human MMP10.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MMP10
Additional Names:
Stromelysin-2, SL-2, Matrix metalloproteinase-10, MMP-10, Transin-2, MMP10, STMY2
Protein Accession Number:
P09238
Protein Gi Number:
116869
Ncbi Gene Id Number:
4319
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP10 degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
Background Reference 1:
Nagase, H., et al., J. Biol. Chem. 274(31):21491-21494 (1999).
Background Reference 2:
Pendas, A.M., et al., Genomics 37(2):266-268 (1996).
Background Reference 3:
Jung, J.Y., et al., Ann. Genet. 33(1):21-23 (1990).
Background Reference 4:
Sirum, K.L., et al., Biochemistry 28(22):8691-8698 (1989).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-167

List Size:
400 ul

List Price:

$495.00

Datasheet
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