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Antibody staining MMP12 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + human lung lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate.
Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse kidney tissue lysates.
Confocal immunofluorescent analysis of MMP12 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Antibody staining MMP12 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

MMP12 Antibody

CATALOG NUMBER: 62-169

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This MMP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the C-terminal region of human MMP12.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MMP12
Additional Names:
Macrophage metalloelastase, MME, Macrophage elastase, ME, hME, Matrix metalloproteinase-12, MMP-12, MMP12, HME
Protein Accession Number:
P39900
Protein Gi Number:
729179
Ncbi Gene Id Number:
4321
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP12 may be involved in tissue injury and remodeling. This protein has significant elastolytic activity. MMP12 can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, wtih small hydrophobic residues (prefereably alanine) occupying P3. The protein is found in alveolar macrophages but not in peripheral blood monocytes. MMP12 can be induced by exposure to lypopolysaccharide, and is inhibited by dexamethasone.
Background Reference 1:
Nar, H., et al., J. Mol. Biol. 312(4):743-751 (2001).
Background Reference 2:
Lang, R., et al., J. Mol. Biol. 312(4):731-742 (2001).
Background Reference 3:
Gronski, T.J. Jr., et al., J. Biol. Chem. 272(18):12189-12194 (1997).
Background Reference 4:
Shapiro, S.D., et al., J. Biol. Chem. 268(32):23824-23829 (1993).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-169

List Size:
400 ul

List Price:

$495.00

Datasheet
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