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Western blot analysis of extracts of various cell lines, using MMP12 antibody (13-885).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse lung using MMP12 antibody (13-885) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using MMP12 antibody (13-885) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using MMP12 antibody (13-885).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

MMP12 Antibody [H7E5]

CATALOG NUMBER: 13-885

Clonality:
Monoclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein of human MMP12
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
A-549
Positive Control 2:
HeLa
Positive Control 3:
MCF7
Positive Control 4:
THP-1
Predicted Molecular Weight:
Observed: 37kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MMP12
Additional Names:
MMP12, MME, MGC138506, matrix metalloproteinase 12 (macrophage elastase), matrix metalloproteinase 12, matrix metallopeptidase 12 (macrophage elastase), macrophage metalloelastase, macrophage elastase, HME
Ncbi Gene Id Number:
4321
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-885

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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