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43-321 (1ug/ml) staining of NIH3T3, (A) A549 (B) and HeLa (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-321 (1ug/ml) staining of NIH3T3, (A) A549 (B) and HeLa (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MMP14 Antibody

CATALOG NUMBER: 43-321

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-ESATPLRFREVP
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in lysates of cell lines NIH3T3, A549 and HeLa (calculated MW of 65.9kDa according to NP_004986.1). Recommended concentration: 1-3ug/ml.
Predicted Molecular Weight:
Approx 60kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MMP14
Additional Names:
MMP14, matrix metallopeptidase 14 (membrane-inserted), 1, MMP-14, MMP-X1, MT-MMP, MT-MMP 1, MT1-MMP, MT1MMP, MTMMP1, matrix metalloproteinase 14 (membrane-inserted), matrix metalloproteinase-14, membrane type 1 metalloprotease, membrane-type matrix metalloproteinase 1, membrane-type-1 matrix metalloproteinase
Protein Accession Number:
NP_004986.1
Protein Gi Number:
317373419
Ncbi Gene Id Number:
4323
Background Reference 1:
Ueda M, Yamashita Y, Takehara M, Terai Y, Kumagai K, Ueki K, Kanda K, Yamaguchi H, Akise D, Hung YC, Ueki M.Survivin gene expression in endometriosis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-321

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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