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Western blot analysis of various lysates using MMP14/MMP14/MT1-MMP Rabbit mAb at 1：1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.
Western blot analysis of various lysates using MMP14/MMP14/MT1-MMP Rabbit mAb at 1：1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using MMP14/MT1-MMP Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using MMP14/MT1-MMP Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using MMP14/MT1-MMP Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Flow cytometry: 1X10^6 MCF7 cells (negative control,left) and HT-1080 (right) cells were intracellularly-stained with MMP14/MT1-MMP Rabbit mAb or PSFlo 488 Rabbit IgG isotype control, followed by Alexa Fluor® 488 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 MCF7 cells (negative control,left) and HT-1080 (right) cells were intracellularly-stained with MMP14/MT1-MMP Rabbit mAb or PSFlo 488 Rabbit IgG isotype control, followed by Alexa Fluor® 488 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 MCF7 cells (negative control,left) and HT-1080 (right) cells were intracellularly-stained with MMP14/MT1-MMP Rabbit mAb or PSFlo 647 Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 MCF7 cells (negative control,left) and HT-1080 (right) cells were intracellularly-stained with MMP14/MT1-MMP Rabbit mAb or PSFlo 647 Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Western blot analysis of various lysates using MMP14/MMP14/MT1-MMP Rabbit mAb at 1：1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.

MMP14/MT1-MMP Rabbit Recombinant Antibody (81-006)

CATALOG NUMBER: 81-006

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IHC-P, WB
Application Note:
WB,1:500 - 1:2000, IHC-P, 1:50 - 1:200, FC, 1:100 - 1:500, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HeLa,HepG2,Mouse lung,Mouse kidney,Mouse heart,Rat heart
Predicted Molecular Weight:
66kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MMP14
Additional Names:
MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1; MMP14/MT1-MMP
Protein Accession Number:
P50281
Ncbi Gene Id Number:
4323
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-006

List Size:
100 uL

List Price:

$477.00

Datasheet
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