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Antibody is used in Western blot to detect MMP15 in mouse brain tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate.
Formalin-fixed and paraffin-embedded human colon carcinoma with MMP15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of WiDr cells using MMP15 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Antibody is used in Western blot to detect MMP15 in mouse brain tissue lysate (lane 1) and HL60 cell lysate (lane 2) lysate.

MMP15 Antibody

CATALOG NUMBER: 62-172

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This MMP15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the N-terminal region of human MMP15.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
76 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MMP15
Additional Names:
Matrix metalloproteinase-15, MMP-15, 3424-, Membrane-type matrix metalloproteinase 2, MT-MMP 2, MTMMP2, Membrane-type-2 matrix metalloproteinase, MT2-MMP, MT2MMP, SMCP-2, MMP15
Protein Accession Number:
P51511
Protein Gi Number:
1705988
Ncbi Gene Id Number:
4324
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP15 is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted.
Background Reference 1:
Jung, M., et al., Prostate 55(2):89-98 (2003).
Background Reference 2:
Nagase, H., et al., J. Biol. Chem. 274(31):21491-21494 (1999).
Background Reference 3:
d'Ortho, M.P., et al., Eur. J. Biochem. 250(3):751-757 (1997).
Background Reference 4:
Sato, H., et al., Genomics 39(3):412-413 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-172

List Size:
400 ul

List Price:

$495.00

Datasheet
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