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Western blot analysis of extracts of various cell lines, using MMP16 antibody (13-634) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human lung cancer using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using MMP16 antibody (13-634) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using MMP16 antibody (13-634) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

MMP16 Antibody

CATALOG NUMBER: 13-634

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 290-565 of human MMP16 (NP_005932.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
U-87MG
Positive Control 2:
HT-29
Positive Control 3:
MCF7
Positive Control 4:
HT-1080
Positive Control 5:
Mouse brain
Positive Control 6:
Rat brain
Predicted Molecular Weight:
Observed: 60kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MMP16
Additional Names:
MMP16, C8orf57, MMP-X2, MT-MMP3, MT3MMP, MT-MMP 3, MT-MMP2, MT2-MMP, Matrix metalloproteinase-16, Membrane type 3-MMP, MMP-16, MMPX2, MT3-MMP, MTMMP3
Ncbi Gene Id Number:
4325
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-634

List Size:
100 uL

List Price:

$436.00

Datasheet
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