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Western blot analysis of hMMP19-R359 in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human breast carcinoma with MMP19 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of K562 cells using MMP19 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of hMMP19-R359 in K562 cell line lysates (35ug/lane)

MMP19 Antibody

CATALOG NUMBER: 62-175

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This MMP19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-373 amino acids from the C-terminal region of human MMP19.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MMP19
Additional Names:
Matrix metalloproteinase-19, MMP-19, 3424-, Matrix metalloproteinase RASI, Matrix metalloproteinase-18, MMP-18, MMP19, MMP18, RASI
Protein Accession Number:
Q99542
Protein Gi Number:
12643345
Ncbi Gene Id Number:
4327
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The function of MMP19 has not been determined. This gene was previously referred to as MMP18 but has been renamed matrix metalloproteinase 19 (MMP19).
Background Reference 1:
Sadowski, T., et al., Mol. Biol. Cell 14(11):4569-4580 (2003).
Background Reference 2:
Impola, U., et al., Int. J. Cancer 103(6):709-716 (2003).
Background Reference 3:
Mauch, S., et al., J. Immunol. 168(3):1244-1251 (2002).
Background Reference 4:
Stracke, J.O., et al., J. Biol. Chem. 275(20):14809-14816 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-175

List Size:
400 ul

List Price:

$495.00

Datasheet
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