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Western blot analysis of extracts of Mouse heart, using MMP3 antibody (14-227) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of C6 cells using MMP3 Polyclonal Antibody (14-227) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using MMP3 Polyclonal Antibody (14-227) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of Mouse heart, using MMP3 antibody (14-227) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

MMP3 Antibody

CATALOG NUMBER: 14-227

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 248-477 of human MMP3 (NP_002413.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
Positive Control 1:
Mouse heart
Predicted Molecular Weight:
Observed: 54kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MMP3
Additional Names:
CHDS6, matrix metallopeptidase 3 (stromelysin 1, progelatinase), matrix metalloproteinase 3 (stromelysin 1, matrix metalloproteinase-3, MGC126102, MGC126103, MGC126104, MMP3, MMP-3, OTTHUMP00000045949, proteoglycan, SL-1, STMY, STMY1, STR1
Ncbi Gene Id Number:
4314
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-227

List Size:
100 uL

List Price:

$436.00

Datasheet
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