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43-572 (1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-572 (0.1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-572 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
43-572 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
43-572 (1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MMP9 Antibody

CATALOG NUMBER: 43-572

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Immunogen:
The immunogen for this antibody is: C-KLDSVFEEPLSKK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx.80kDa and 70kDa bands observed in Human Peripheral Blood Mononucleocytes lysates. These bands correspond to the mature forms (http://www.uniprot.org/uniprot/P14780). This protein has a calculated MW of 78.5kDa according to NP_004985.2, but the precursor is usually seen as 92kDa or larger. Recommended concentration: 1-3ug/ml.Approx.75kDa band observed in Mouse Heart lysates (calculated MW of 80.5kDa according to Mouse NP_038627.1). Recommended concentration: 0.1-0.3ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MMP9
Additional Names:
MMP9, matrix metalloproteinase 9, gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase, HGNC:7176, CLG4B, GELB, 92kD type IV collagenase, gelatinaseB, macrophage gelatinase, matrix metalloproteinase 9 (gelatinase B, 92kD gelatinase, 92kD type IV collagenase), type V collagenase
Protein Accession Number:
NP_004985.2
Protein Gi Number:
74272287
Ncbi Gene Id Number:
4318
Background Reference 1:
Gurer G, Erdem S, Kocaefe C, Ozguc M, Tan E. Expression of matrix metalloproteinases in vasculitic neuropathy.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-572

List Size:
0.1 mg

List Price:

$469.00

Out of stock

Datasheet
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