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Western blot analysis of MMP9 in mouse lung tissue lysate with MMP9 antibody at 1 μg/ml.
Immunohistochemistry of MMP9 in human lung tissue with MMP9 antibody at 2.5 μg/mL.
Immunofluorescence of MMP9 in human lung tissue with MMP9 antibody at 20 μg/mL.
Western blot analysis of MMP9 in mouse lung tissue lysate with MMP9 antibody at 1 μg/ml.
Trial Size

MMP9 Antibody

CATALOG NUMBER: 7471

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%)
Immunogen:
MMP9 antibody was raised against a 16 amino acid peptide near the center of human MMP9.
The immunogen is located within amino acids 260 - 310 of MMP9 .
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MMP9 antibody can be used for detection of MMP9 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
MMP9 antibody is human, mouse and rat reactive. At least three isoforms of MMP9 are known to exist; this antibody only recognizes the two longest isoforms.
Positive Control 1:
Cat. No. 1402 - Mouse Lung Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
Predicted: 76 kDa
Observed: 73kDa
Purification:
MMP9 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MMP9 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MMP9 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MMP9
Additional Names:
MMP9 Antibody : GELB, CLG4B, MMP-9, MANDP2, Matrix metalloproteinase-9, 92 kDa gelatinase
Protein Accession Number:
NP_004985
Protein Gi Number:
74272287
Ncbi Gene Id Number:
4318
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The matrix metalloproteinase (MMP) family are a family of proteins that are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases (1). MMP9 degrades type IV and V collagens (2) and studies suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow (3) and plays a role in tumor-associated tissue remodeling (4).
Background Reference 1:
Iyer RP, Patterson NL, Fields GB, et al. The history of matrix metalloproteinases: milestones, myths, and misperceptions. Am. J. Heart Circ. Physiol 2012; 303:H919-30.
Background Reference 2:
Murphy G, Knauper V, Atkinson S, et al. Matrix metalloproteinases in arthritic disease. Arthritis Res. 2002; 4 Suppl. 3:S39-49.
Background Reference 3:
Sweeney EA, Lortat-Jacob H, Priestley GV, et al. Sulfated polysaccharides increase plasma levels of SDF-1 in monkeys and mice: involvement in mobilization of stem/progenitor cells. Blood 2002; 99:44-51.
Background Reference 4:
Rhee JW, Lee KW, Sohn WJ, et al. Regulation of matrix metalloproteinase-9 gene expression and cell migration by NF-kappa B in response to CpG-oligodeoxynucleotides in RAW 264.7 cells. Mol. Immunol. 2007; 44:1393-400.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7471

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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