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Western blot analysis of lysates from U-2 OS cells using MMP9 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours. U-2 OS cells were treated with PMA (200 nM) at 37℃ for 48 hours after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using MMP9 Rabbit mAb at a dilution of 1:20000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using MMP9 Rabbit mAb at a dilution of 1:20000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung tissue using MMP9 Rabbit mAb at a dilution of 1:20000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MMP9 Rabbit mAb at a dilution of 1:20000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Human tonsil tissue using MMP9 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Human spleen tissue using MMP9 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Western blot analysis of lysates from U-2 OS cells using MMP9 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours. U-2 OS cells were treated with PMA (200 nM) at 37℃ for 48 hours after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20s.

MMP9 Rabbit Recombinant Antibody (83-596)

CATALOG NUMBER: 83-596

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, IHC-P, WB
Application Note:
WB,1:5000 - 1:20000, IF-P,1:300 - 1:1500, IHC-P,1:5000 - 1:20000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
U-2 OS treated with PMA
Predicted Molecular Weight:
78kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MMP9
Additional Names:
GELB; CLG4B; MMP-9; MANDP2
Protein Accession Number:
P14780
Ncbi Gene Id Number:
4318
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-596

List Size:
100 uL

List Price:

$477.00

Datasheet
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