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Western blot analysis of MMS21 in 293 cell lysate with MMS21 antibody at (A) 0.5 and (B) 1 μg/mL.
Western blot analysis of MMS21 in 293 cell lysate with MMS21 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

MMS21 Antibody

CATALOG NUMBER: 5759

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (80%)
Immunogen:
MMS21 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human MMS21.
The immunogen is located within amino acids 170 - 220 of MMS21.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
MMS21 antibody can be used for detection of MMS21 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Predicted Molecular Weight:
Predicted: 27 kDa
Observed: 30 kDa
Purification:
MMS21 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MMS21 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MMS21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NSMCE2
Additional Names:
MMS21 Antibody: NSE2, MMS21, ZMIZ7, C8orf36, E3 SUMO-protein ligase NSE2, MMS21 homolog, hMMS21
Protein Accession Number:
NP_775956
Protein Gi Number:
27734761
Ncbi Gene Id Number:
286053
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MMS21 Antibody: MMS21, also known as NSE2, is a SUMO ligase that in combination with the SMC5/6 complex is required for the prevention of DNA damage induced apoptosis by facilitating DNA repair in human cells. MMS21-dependent sumoylation is integral and important to the cohesion mechanism and mitotic progression; this function appears to be independent of SMC6. MMS21 mediates SUMO attachment to various proteins such as SMC6L1 and TRAX, and possibly the cohesin components RAD21 and STAG2.
Background Reference 1:
Potts PR and Yu H. Human MMS21/NSE2 is a SUMO ligase required for DNA repair. Mol. Cell. Biol. 2005; 25:7021-32.
Background Reference 2:
Behlke-Steinert S, Touat-Todeschini L, Skoufias DA, et al. SMC5 and MMS21 are required for chromosome cohesion and mitotic progression. Cell Cycle 2009; 8:2211-8.
Background Reference 3:
Bermudez-Lopez M, Ceschia A, de Piccoli G, et al. The Smc5/6 complex is required for dissolution of DNA-mediated sister chromatid linkages. Nucleic Acids Res. 2010; 38:6502-12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5759

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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