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Western blot analysis of extracts of various cell lines, using MNAT1 antibody (18-384) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug MNAT1 antibody (18-384). Western blot was performed from the immunoprecipitate using MNAT1 antibody (18-384) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using MNAT1 antibody (18-384) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

MNAT1 Antibody

CATALOG NUMBER: 18-384

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MNAT1 (NP_002422.1).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:50 - 1:200
Positive Control 1:
PC-3
Positive Control 2:
22Rv1
Positive Control 3:
DU 145
Positive Control 4:
293T
Positive Control 5:
Mouse testis
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 36kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MNAT1
Additional Names:
MNAT1, MAT1, TFB3, CAP35, RNF66
Ncbi Gene Id Number:
4331
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-384

List Size:
100 uL

List Price:

$436.00

Datasheet
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