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42-837 (0.02ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-837 (5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-837 (0.02ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MOB2 Antibody

CATALOG NUMBER: 42-837

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: DERGKKVKCTAPQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 30.2kDa according to NP_001165694.1). Recommended concentration: 0.02-0.06ug/ml.An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Lung shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole. Recommended concentration, 5-10ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_001165694.1; NP_443731.2) only.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MOB2
Additional Names:
MOB2, HCCA2, HCCA2 protein, mob2 homolog, mps one binder kinase activator-like 2
Protein Accession Number:
NP_001165694.1,NP_443731.2
Protein Gi Number:
288557345
Ncbi Gene Id Number:
81532

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-837

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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