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Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody at 1:1000 at each lane.
Western blot analysis in Daudi,K562 cell line lysates (35ug/lane).
Fluorescent image of NIH/3T3 cell stained with MOB4A Antibody .NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MOB4A primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).MOB4A immunoreactivity is localized to Cytoplasm significantly.
Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody at 1:1000 at each lane.

MOB4A Antibody

CATALOG NUMBER: 62-608

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This MOB4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the C-terminal region of human MOB4A.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
25 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MOB1B
Additional Names:
MOB kinase activator 1B, Mob1 homolog 1A, Mob1A, Mob1B, Mps one binder kinase activator-like 1A, MOB1B, MOB4A, MOBKL1A
Protein Accession Number:
Q7L9L4
Protein Gi Number:
56749324
Ncbi Gene Id Number:
92597
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MOB4A is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Mob4A regulates NDR1 and NDR2 kinase activity, widely expressed nuclear serine-threonine kinases that have been implicated in cell proliferation and/or tumor progression.
Background Reference 1:
Bichsel,S.J.,J. Biol. Chem. 279 (34), 35228-35235 (2004) Devroe,E.,J. Biol. Chem. 279 (23), 24444-24451 (2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-608

List Size:
400 ul

List Price:

$519.00

Datasheet
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