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42-455 (0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-455 (5ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-455 (0.3ug/ml) staining of Mouse Brain lysate (A) and (1ug/ml) staining of Rat Brain lysate (B) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-455 (0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

MOBKL3 Antibody

CATALOG NUMBER: 42-455

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NSNKYFPSRVSIKES
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 26kDa band observed in Human Brain (Frontal Cortex) and in Mouse and Rat Brain lysates (calculated MW of 26.0kDa according to NP_056202.2). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in the neuronal bodies. Recommended concentration, 5-10ug/ml.
Specificity:
This antibody is expected to recognize all three reported isoforms (NP_056202.2; NP_955776.1; NP_001094289.1).
Positive Control 1:
Cat. No. 1366 - Human Frontal Lobe Tissue Lysate
Positive Control 2:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 3:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MOBKL3
Additional Names:
MOBKL3, MOB1, Mps One Binder kinase activator-like 3 (yeast), 2C4D, MGC12264, MOB1, MOB3, PREI3, class II mMOB1, mob1 homolog 3, mps one binder kinase activator-like 3, phocein, preimplantation protein 3
Protein Accession Number:
NP_056202.2,NP_955776.1,NP_001094289.1
Protein Gi Number:
41349449
Ncbi Gene Id Number:
25843

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-455

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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