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Western blot analysis of extracts of mouse liver, using MOK antibody (18-140) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse kidney using MOK antibody (18-140) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using MOK antibody (18-140) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using MOK antibody (18-140) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of mouse liver, using MOK antibody (18-140) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

MOK Antibody

CATALOG NUMBER: 18-140

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MOK (NP_055041.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Mouse liver
Predicted Molecular Weight:
Observed: 40kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
MOK
Additional Names:
MAPK/MAK/MRK overlapping kinase, MOK protein kinase, Renal tumor antigen 1, RAGE-1, MOK, RAGE, RAGE1
Ncbi Gene Id Number:
5891
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene belongs to the MAP kinase superfamily. The gene was found to be regulated by caudal type transcription factor 2 (Cdx2) protein. The encoded protein, which is localized to epithelial cells in the intestinal crypt, may play a role in growth arrest and differentiation of cells of upper crypt and lower villus regions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-140

List Size:
100 uL

List Price:

$436.00

Datasheet
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