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45-896 (0.5ug/ml) staining of Human Frontal Cortex (A) and Mouse Adipose (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-896 (5ug/ml) staining of paraffin embedded Human Liver. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
45-896 Negative Control showing staining of paraffin embedded Human Liver, with no primary antibody.
45-896 (0.5ug/ml) staining of Human Frontal Cortex (A) and Mouse Adipose (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Monoglyceride Lipase Antibody

CATALOG NUMBER: 45-896

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QDLPHLVNADGQY
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human Brain (Frontal Cortex) and Mouse Adipose lysates (calculated MW of 33.3kDa according to NP_001003794.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat for this particular batch.Immunohistochemistry:In paraffin embedded Human Liver, expression of MGLL is detected in hepatocytes. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_009214.1; NP_001003794.1).
Positive Control 1:
Cat. No. XBL-11048 - Human Adipose Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
MGLL
Additional Names:
monoglyceride lipase, HU-K5, MGLL, MGL, lysophospholipase-like
Protein Accession Number:
NP_009214.1,NP_001003794.1
Protein Gi Number:
6005786
Ncbi Gene Id Number:
11343
Background Reference 1:
Blankman JL, Simon GM, Cravatt BF. A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol. Chem Biol. 2007 Dec;14(12):1347-56

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-896

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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