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Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Mouse Aak1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse bladder tissue lysates (35ug/lane).

Mouse Aak1 Antibody

CATALOG NUMBER: 57-852

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Human, Pig, Rat
Immunogen:
This Mouse Aak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279-306 amino acids from the Central region of mouse Aak1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
103 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Aak1
Additional Names:
AP2-associated protein kinase 1, Adaptor-associated kinase 1, Aak1, Kiaa1048
Protein Accession Number:
Q3UHJ0
Ncbi Gene Id Number:
269774
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Aak1 phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis (By similarity).
Background Reference 1:
Munton, R.P., et al. Mol. Cell Proteomics 6(2):283-293(2007)
Background Reference 2:
Nishimura, M., et al. DNA Res. 11(5):315-323(2004)
Background Reference 3:
Jha, A., et al. J. Biol. Chem. 279(3):2281-2290(2004)
Background Reference 4:
Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-852

List Size:
400 ul

List Price:

$495.00

Datasheet
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