Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis in mouse heart tissue lysates (35ug/lane).
Mouse Akt1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse gallbladder tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse heart tissue lysates (35ug/lane).

Mouse Akt1 Antibody

CATALOG NUMBER: 57-924

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Human
Immunogen:
This Mouse Akt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-49 amino acids from the N-terminal region of mouse Akt1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
56 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Akt1
Additional Names:
RAC-alpha serine/threonine-protein kinase, AKT1 kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, Thymoma viral proto-oncogene, Akt1, Akt, Rac
Protein Accession Number:
P31750
Ncbi Gene Id Number:
11651
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the founding member of the Aktserine-threonine protein kinase gene family that also includes Akt2and Akt3. This kinase is a major downstream effector of thephosphatidylinositol 3-kinase (PI3K) pathway that mediates theeffects of various growth factors such as platelet-derived growthfactor (PDGF), epidermal growth factor (EGF), insulin andinsulin-like growth factor I (IGF-I). It is activated throughrecruitment to cellular membranes by PI3K lipid products and byphosphorylation by 3-phosphoinositide dependent kinase-1. It thenfurther phosphorylates different downstream proteins in response tovarious extracellular signals and thus plays a pivotal role inmediating a variety of cellular processes, such as glucosemetabolism, glycogen biosynthesis, protein synthesis and turn over,inflammatory response, cell survival (anti-apoptosis) anddevelopment. Alternatively spliced transcript variants encodingdifferent isoforms have been found for this gene. [provided byRefSeq].
Background Reference 1:
Chang, Z., et al. Dev. Biol. 347(2):384-391(2010)
Background Reference 2:
Zhou, F., et al. Am. J. Pathol. 177(4):2124-2133(2010)
Background Reference 3:
Sun, X., et al. Proc. Natl. Acad. Sci. U.S.A. 107(39):16887-16892(2010)
Background Reference 4:
Goncalves, M.D., et al. PLoS ONE(9), E12707 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-924

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369