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Recombinant Mouse c-Kit ligand/KITLG/SCF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Mouse c-Kit ligand/KITLG/SCF Protein is greater than 95% as determined by SEC-HPLC.
Recombinant Mouse SCF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED<sub>50</sub> for this effect is 0.5-1.0 ng/mL, corresponding to a specific activity of 1.0×10<sup>6</sup>-2.0×10<sup>6</sup> units/mg.
Immobilized recombinant Mouse SCF at 2 μg/mL (100 μL/well) can bind recombinant human CD117 with a linear range of 1.5-10ng/mL.
Recombinant Mouse c-Kit ligand/KITLG/SCF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Mouse c-Kit ligand/KITLG/SCF Recombinant Protein (85-211)

CATALOG NUMBER: 85-211

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Mouse
source-species:
HEK293 cells
Recombinant Protein Sequence:
Lys26-Ala189
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
19.14 kDa
Biological Activity:
1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.5-1.0 ng/mL, corresponding to a specific activity of 1.0×106-2.0×106 units/mg.
2. Measured by its binding ability in a functional ELISA.Immobilized recombinant Mouse SCF at 2 μg/mL (100 μL/well) can bind recombinant human CD117 with a linear range of 1.5-10 ng/mL.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
KITLG,FPH2,KL-1,Kitl,MGF,SCF,SF,SHEP7,KL,SCF/KITLG
Protein Accession Number:
NP_038626.1.
Ncbi Gene Id Number:
17311

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-211

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
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