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Western blot analysis in mouse testis tissue lysates (35ug/lane).
Mouse Dapk3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse testis tissue lysates (35ug/lane).

Mouse Dapk3 Antibody

CATALOG NUMBER: 57-930

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Mouse Dapk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-282 amino acids from the Central region of mouse Dapk3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
51 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Dapk3
Additional Names:
Death-associated protein kinase 3, DAP kinase 3, DAP-like kinase, Dlk, MYPT1 kinase, ZIP-kinase, Dapk3, Zipk
Protein Accession Number:
O54784
Ncbi Gene Id Number:
13144
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection (By similarity).
Background Reference 1:
Chang, A.N., et al. J. Biol. Chem. 285(8):5122-5126(2010)
Background Reference 2:
Ohbayashi, N., et al. Biochem. Biophys. Res. Commun. 372(4):708-712(2008)
Background Reference 3:
Wooldridge, A.A., et al. J. Biol. Chem. 283(17):11850-11859(2008)
Background Reference 4:
Shoval, Y., et al. PLoS Genet. 3(10):1884-1893(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-930

List Size:
400 ul

List Price:

$495.00

Datasheet
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