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Western blot analysis of anti-mDnmt2 Pab in HL-60 cell lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-mDnmt2 Pab in HL-60 cell lysate

Mouse Dnmt2 Antibody

CATALOG NUMBER: 55-291

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Mouse Dnmt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from the N-terminal region of mouse Dnmt2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Trdmt1
Additional Names:
tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog MmuIIP, DNA MTase homolog MmuIIP, MMmuIIP, Met-2, Trdmt1, Dnmt2, Met2
Protein Accession Number:
O55055
Ncbi Gene Id Number:
13434
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
mDnmt2 does not appear to be active as a DNA methyltransferase; however, its strong binding to DNA suggests that it may mark specific sequences in the genome by binding to DNA through the specific target-recognizing motif. mDnmt2 is strongly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells. This protein belongs to the 5-cystosine methyltransferase family.
Background Reference 1:
Yoder, J.A., et al., Hum. Mol. Genet. 7(2):279-284 (1998).
Background Reference 2:
Okano, M., et al., Nucleic Acids Res. 26(11):2536-2540 (1998).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-291

List Size:
400 ul

List Price:

$495.00

Datasheet
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