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Western blot analysis of Mouse Dnmt3L Antibody in Hela cell line lysates (35ug/lane)
Western blot analysis in mouse liver tissue lysates (35ug/lane).
Western blot analysis of Mouse Dnmt3L Antibody in Hela cell line lysates (35ug/lane)

Mouse Dnmt3L Antibody

CATALOG NUMBER: 55-295

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Mouse Dnmt3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-421 amino acids from mouse Dnmt3L.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Dnmt3l
Additional Names:
DNA (cytosine-5)-methyltransferase 3-like, Dnmt3l
Protein Accession Number:
Q9CWR8
Ncbi Gene Id Number:
54427
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
mDnmt3L (DNA methyltransferase 3L) does not appear to be catalytically active due to loss of the critical active site residues. It may function not directly as a DNA methyltransferase but rather as a regulator of methylation at imprinted loci. It is required specifically for the establishment of genomic imprints but is dispensable for their propagation. It is essential for the de novo methylation of single-copy DNA sequences. The protein is well-express in testis, thymus, ovary, and heart. mDnmt3L, which belongs to the 5-cytosine methyltransferase family, contains 1 ADD-type zinc finger.
Background Reference 1:
Okazaki, Y., et al., Nature 420(6915):563-573 (2002).
Background Reference 2:
Bourc'his, D., et al., Science 294(5551):2536-2539 (2001).
Background Reference 3:
Aapola, U., et al., Cytogenet. Cell Genet. 92 (1-2), 122-126 (2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-295

List Size:
400 ul

List Price:

$495.00

Datasheet
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