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Western blot analysis in K562 cell line lysates (35ug/lane).
Mouse Epha4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in K562 cell line lysates (35ug/lane).

Mouse Epha4 Antibody

CATALOG NUMBER: 57-350

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Mouse Epha4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 464-492 amino acids from the Central region of mouse Epha4.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
110 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Epha4
Additional Names:
Ephrin type-A receptor 4, Tyrosine-protein kinase receptor MPK-3, Tyrosine-protein kinase receptor SEK-1, Epha4, Sek, Sek1
Protein Accession Number:
Q03137
Ncbi Gene Id Number:
13838
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in a signal transduction process involved in hindbrain pattern formation.
Background Reference 1:
Kuwako, K., et al. Neuron 67(3):407-421(2010)
Background Reference 2:
Qu, Y., et al. J. Neurosci. 30(28):9392-9401(2010)
Background Reference 3:
Oginuma, M., et al. Development 137(9):1515-1522(2010)
Background Reference 4:
Xie, Z., et al. Proc. Natl. Acad. Sci. U.S.A. 107(14):6510-6515(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-350

List Size:
400 ul

List Price:

$495.00

Datasheet
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