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Western blot analysis in CEM cell line lysates (35ug/lane).This demonstratedetected the Hck protein (arrow).
Western blot analysis in CEM cell line lysates (35ug/lane).This demonstratedetected the Hck protein (arrow).

Mouse Hck Antibody

CATALOG NUMBER: 58-266

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Mouse Hck antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-101 amino acids from the N-terminal region of mouse Hck.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Hck
Additional Names:
Tyrosine-protein kinase HCK, B-cell/myeloid kinase, BMK, Hematopoietic cell kinase, Hemopoietic cell kinase, p56-HCK/p59-HCK, Hck
Protein Accession Number:
P08103
Ncbi Gene Id Number:
15162
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the Srcfamily of tyrosine kinases. This protein is primarily hemopoietic,particularly in cells of the myeloid and B-lymphoid lineages. Itmay play a role in the innate immune response and the STAT5signaling pathway. Alternative translation initiation site usage,including a non-AUG (CUG) codon, results in the production of twodifferent isoforms, that have different subcellular localization.
Background Reference 1:
Verollet, C., et al. J. Immunol. 184(12):7030-7039(2010)
Background Reference 2:
Ruschmann, J., et al. Exp. Hematol. 38(5):392-402(2010)
Background Reference 3:
Lowell, C.A., et al. J. Cell Biol. 133(4):895-910(1996)
Background Reference 4:
Lowell, C.A., et al. Blood 87(5):1780-1792(1996)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-266

List Size:
400 ul

List Price:

$495.00

Datasheet
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