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Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).

Mouse Irak1 Antibody

CATALOG NUMBER: 57-735

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
This Mouse Irak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-433 amino acids from the Central region of mouse Irak1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
77 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Irak1
Additional Names:
Interleukin-1 receptor-associated kinase 1, IRAK, IRAK-1, Pelle-like protein kinase, mPLK, Irak1, Il1rak
Protein Accession Number:
Q62406
Ncbi Gene Id Number:
16179
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Irak1 binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. The kinase activity of this enzyme may not be required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
Background Reference 1:
Vaughan, T., et al. Mol. Immunol. 47(15):2515-2518(2010)
Background Reference 2:
Hoshino, K., et al. J. Immunol. 184(7):3341-3345(2010)
Background Reference 3:
Dong, J.W., et al. Am. J. Physiol. Heart Circ. Physiol. 298 (3), H1079-H1087 (2010) :
Background Reference 4:
Gan, L., et al. Mol. Immunol. 47(6):1278-1282(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-735

List Size:
400 ul

List Price:

$495.00

Datasheet
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