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Western blot analysis in mouse liver tissue lysates (35ug/lane).
Western blot analysis in mouse liver tissue lysates (35ug/lane).

Mouse Irf3 Antibody

CATALOG NUMBER: 60-856

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig
Immunogen:
This Mouse Irf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids from the N-terminal region of mouse Irf3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Irf3
Additional Names:
Interferon regulatory factor 3, IRF-3, Irf3
Protein Accession Number:
P70671
Ncbi Gene Id Number:
54131
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains (By similarity).
Background Reference 1:
Marichal, T., et al. J. Allergy Clin. Immunol. 126(4):836-844(2010)
Background Reference 2:
Menachery, V.D., et al. J. Virol. 84(19):9685-9694(2010)
Background Reference 3:
Carrigan, S.O., et al. J. Immunol. 185(6):3602-3609(2010)
Background Reference 4:
Wang, J., et al. J. Immunol. 185(3):1720-1729(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-856

List Size:
400 ul

List Price:

$495.00

Datasheet
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