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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Western blot analysis of Map2k1 using rabbit polyclonal Mouse Map2k1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k1 gene.
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
Mouse Map2k1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

Mouse Map2k1 Antibody

CATALOG NUMBER: 57-516

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Hamster, Rabbit, Rat
Immunogen:
This Mouse Map2k1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-296 amino acids from the C-terminal region of mouse Map2k1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Map2k1
Additional Names:
Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, Map2k1, Mek1, Prkmk1
Protein Accession Number:
P31938
Ncbi Gene Id Number:
26395
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Map2k1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Background Reference 1:
Chandrakesan, P., et al. J. Biol. Chem. 285(43):33485-33498(2010)
Background Reference 2:
Yang, T., et al. J. Clin. Invest. 120(7):2474-2485(2010)
Background Reference 3:
Cho, K.W., et al. J. Exp. Zool.Mol. Dev. Evol. 312(8):901-911(2009)
Background Reference 4:
Jo, C., et al. Cell. Signal. 21(12):1910-1917(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-516

List Size:
400 ul

List Price:

$495.00

Datasheet
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