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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis of Map2k3 using rabbit polyclonal Mouse Map2k3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k3 gene.
Western blot analysis in C6 cell line lysates (35ug/lane).
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

Mouse Map2k3 Antibody

CATALOG NUMBER: 57-736

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Mouse Map2k3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-160 amino acids from the Central region of mouse Map2k3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Map2k3
Additional Names:
Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, MEK 3, Map2k3, Mkk3, Prkmk3
Protein Accession Number:
O09110
Ncbi Gene Id Number:
26397
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38 (By similarity).
Background Reference 1:
Yang, Z., et al. J. Immunol. 185(10):6205-6213(2010)
Background Reference 2:
Remy, G., et al. Cell. Signal. 22(4):660-667(2010)
Background Reference 3:
Behren, A., et al. Oncogene 29(10):1519-1530(2010)
Background Reference 4:
Nagelin, M.H., et al. J. Biol. Chem. 284(45):31303-31314(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-736

List Size:
400 ul

List Price:

$495.00

Datasheet
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