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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Western blot analysis of Map2k5 using rabbit polyclonal Mouse Map2k5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k5 gene.
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

Mouse Map2k5 Antibody

CATALOG NUMBER: 57-517

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Mouse Map2k5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 385-412 amino acids from the C-terminal region of mouse Map2k5.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Map2k5
Additional Names:
Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, Map2k5, Mek5, Mkk5, Prkmk5
Protein Accession Number:
Q9WVS7
Ncbi Gene Id Number:
23938
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Map2k5 acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
Background Reference 1:
Spiering, D., et al. J. Biol. Chem. 284(37):24972-24980(2009)
Background Reference 2:
Carter, E.J., et al. J. Cell. Sci. 122 (PT 17), 3104-3112 (2009) :
Background Reference 3:
Sohn, S.J., et al. EMBO J. 27(13):1896-1906(2008)
Background Reference 4:
Shishido, T., et al. Circ. Res. 102(11):1416-1425(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-517

List Size:
400 ul

List Price:

$495.00

Datasheet
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