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Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).
Western blot analysis in mouse brain tissue lysates (35ug/lane).
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).

Mouse Map4k4 Antibody

CATALOG NUMBER: 57-749

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Mouse Map4k4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the Central region of mouse Map4k4.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
141 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Map4k4
Additional Names:
Mitogen-activated protein kinase kinase kinase kinase 4, HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, MEK kinase kinase 4, MEKKK 4, Nck-interacting kinase, Map4k4, Nik
Protein Accession Number:
P97820
Ncbi Gene Id Number:
26921
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway.
Background Reference 1:
Maruyama, T., et al. J. Bone Miner. Res. 25(5):1058-1067(2010)
Background Reference 2:
Guntur, K.V., et al. J. Biol. Chem. 285(9):6595-6603(2010)
Background Reference 3:
Aouadi, M., et al. Nature 458(7242):1180-1184(2009)
Background Reference 4:
Wittwer, T., et al. Biochem. Biophys. Res. Commun. 371(2):294-297(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-749

List Size:
400 ul

List Price:

$495.00

Datasheet
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