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Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstratedetected the Nlk protein (arrow).
Western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstratedetected the Nlk protein (arrow).
Mouse Nlk Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstratedetected the Nlk protein (arrow).

Mouse Nlk Antibody

CATALOG NUMBER: 57-359

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This Mouse Nlk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 408-436 amino acids from the C-terminal region of mouse Nlk.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Nlk
Additional Names:
Serine/threonine-protein kinase NLK, Nemo-like kinase, Nlk {ECO:0000312, EMBL:AAC244991}
Protein Accession Number:
O54949
Ncbi Gene Id Number:
18099
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
Background Reference 1:
Li, M., et al. J. Biol. Chem. 285(18):13397-13404(2010)
Background Reference 2:
Nifuji, A., et al. Exp. Cell Res. 316(7):1127-1136(2010)
Background Reference 3:
Ishitani, T., et al. Nat. Cell Biol. 12(3):278-285(2010)
Background Reference 4:
Martinez, G., et al. Invest. Ophthalmol. Vis. Sci. 50(10):4794-4806(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-359

List Size:
400 ul

List Price:

$495.00

Datasheet
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