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Western blot analysis in mouse liver tissue lysates (35ug/lane).
Mouse Pdk3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse liver tissue lysates (35ug/lane).

Mouse Pdk3 Antibody

CATALOG NUMBER: 57-611

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
This Mouse Pdk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-414 amino acids from the C-terminal region of mouse Pdk3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Pdk3
Additional Names:
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial, Pyruvate dehydrogenase kinase isoform 3, Pdk3
Protein Accession Number:
Q922H2
Ncbi Gene Id Number:
236900
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Pdk3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (By similarity).
Background Reference 1:
Pagliarini, D.J., et al. Cell 134(1):112-123(2008)
Background Reference 2:
Blackshaw, S., et al. PLoS Biol.(9), E247 (2004) :
Background Reference 3:
Visel, A., et al. Nucleic Acids Res. 32 (DATABASE ISSUE), D552-D556 (2004) :
Background Reference 4:
Mootha, V.K., et al. Cell 115(5):629-640(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-611

List Size:
400 ul

List Price:

$495.00

Datasheet
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