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Western blot analysis in Ramos cell line lysates (35ug/lane).
Western blot analysis in mouse stomach tissue lysates (35ug/lane).
Flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Ramos cell line lysates (35ug/lane).

Mouse Phkg1 Antibody

CATALOG NUMBER: 57-595

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Mouse Phkg1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-331 amino acids from the C-terminal region of mouse Phkg1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Phkg1
Additional Names:
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform, Phosphorylase kinase subunit gamma-1, Serine/threonine-protein kinase PHKG1, Phkg1, Phkg
Protein Accession Number:
P07934
Ncbi Gene Id Number:
18682
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I.
Background Reference 1:
Suto, J., et al. J. Vet. Med. Sci. 66(9):1033-1038(2004)
Background Reference 2:
Visel, A., et al. Nucleic Acids Res. 32 (DATABASE ISSUE), D552-D556 (2004) :
Background Reference 3:
Takenaka, M., et al. Kidney Int. 53(3):562-572(1998)
Background Reference 4:
Maichele, A.J., et al. Mamm. Genome 5(1):15-18(1994)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-595

List Size:
400 ul

List Price:

$495.00

Datasheet
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