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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
Western blot analysis of Sgk3 using rabbit polyclonal Mouse Sgk3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Sgk3 gene.
Western blot analysis in mouse bladder tissue lysates (35ug/lane).
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).

Mouse Sgk3 Antibody

CATALOG NUMBER: 57-630

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Mouse Sgk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-409 amino acids from the C-terminal region of mouse Sgk3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
57 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Sgk3
Additional Names:
Serine/threonine-protein kinase Sgk3, Cytokine-independent survival kinase, Serum/glucocorticoid-regulated kinase 3, Serum/glucocorticoid-regulated kinase-like, Sgk3, Cisk, Sgkl
Protein Accession Number:
Q9ERE3
Ncbi Gene Id Number:
170755
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Sgk3 is involved in the activation of potassium channels (By similarity). Mediates cell IL-3-dependent survival signals. Can inhibit pro-apoptotic FOXO3A in vitro.
Background Reference 1:
Zemtsova, I.M., et al. Am. J. Physiol., Cell Physiol. 299 (5), C1007-C1014 (2010) :
Background Reference 2:
Mauro, T.M., et al. FASEB J. 23(9):3193-3202(2009)
Background Reference 3:
Xu, J., et al. J. Biol. Chem. 284(21):14377-14385(2009)
Background Reference 4:
Campagna, D.R., et al. J. Invest. Dermatol. 128(3):730-732(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-630

List Size:
400 ul

List Price:

$495.00

Datasheet
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