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Fluorescent image of A549 cells stained with XAF1 Mouse Stk11 Antibody . AP17316b was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded M.skeletal muscle section using Mouse Stk11 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysate from NCI-H1299 cell line, using Mouse Stk11 Antibody at 1:1000.
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
Fluorescent image of A549 cells stained with XAF1 Mouse Stk11 Antibody . AP17316b was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Mouse Stk11 Antibody

CATALOG NUMBER: 59-109

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
This Mouse Stk11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-420 amino acids from the C-terminal region of mouse Stk11.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IF starting dilution is: 1:25
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:500-1:1000
Predicted Molecular Weight:
49 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Stk11
Additional Names:
Serine/threonine-protein kinase STK11, Liver kinase B1 homolog, LKB1, mLKB1, Stk11 {ECO:0000312, MGI:MGI:1341870}
Protein Accession Number:
Q9WTK7
Ncbi Gene Id Number:
20869
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases (By similarity). Tumor suppressor.
Background Reference 1:
Gao, Y., et al. Proc. Natl. Acad. Sci. U.S.A. 107(44):18892-18897(2010)
Background Reference 2:
Bungard, D., et al. Science 329(5996):1201-1205(2010)
Background Reference 3:
Koh, H.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(35):15541-15546(2010)
Background Reference 4:
Foretz, M., et al. J. Clin. Invest. 120(7):2355-2369(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-109

List Size:
400 ul

List Price:

$495.00

Datasheet
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