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Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstratedetected the Syk protein (arrow).
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstratedetected the Syk protein (arrow).

Mouse Syk Antibody

CATALOG NUMBER: 58-297

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Rat
Immunogen:
This Mouse Syk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the Central region of mouse Syk.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
71 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Syk
Additional Names:
Tyrosine-protein kinase SYK, Spleen tyrosine kinase, Syk, ptk72, Sykb
Protein Accession Number:
P48025
Ncbi Gene Id Number:
20963
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Positive effector of BCR-stimulated responses. Couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion. Phosphorylates USP25 and regulates its intracellular levels (By similarity).
Background Reference 1:
Heizmann, B., et al. Proc. Natl. Acad. Sci. U.S.A. 107(43):18563-18568(2010)
Background Reference 2:
Fernandez, P.L., et al. J. Biol. Chem. 285(43):32844-32851(2010)
Background Reference 3:
Zou, W., et al. J. Cell. Sci. 123 (PT 17), 2955-2963 (2010) :
Background Reference 4:
Falet, H., et al. J. Exp. Med. 207(9):1967-1979(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-297

List Size:
400 ul

List Price:

$495.00

Datasheet
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