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Mouse TGF-alpha Recombinant Protein (85-840)

CATALOG NUMBER: 85-840

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Mouse
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ala38-Ala88
Fusion Tag:
C-hFc
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
32.08 kDa
Biological Activity:
Measured in a cell proliferation assay using Balb3T3 mouse fibroblast cells. The ED50 for this effect is 0.43-1.72 ng/mL, corresponding to a specific activity of 15.81×105~2.33×106 units/mg.
Purity:
≥ 85% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
Protransforming growth factor alpha, Cleaved into: Transforming growth factor alpha, TGF-alpha, EGF-like TGF, ETGF, TGF type 1, Tgfa
Protein Accession Number:
NP_112476.1
Ncbi Gene Id Number:
21802
Background:
Transforming growth factor alpha (TGF-α) is a protein that in humans is encoded by the TGFA gene. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes, and macrophages.TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-840

List Size:
0.01 mg

List Price:

$140.00

Datasheet
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