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Western blot analysis of Mouse TLR6 Antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Mouse TLR6 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of Mouse TLR6 Antibody in K562 cell line lysates (35ug/lane)

Mouse TLR6 Antibody

CATALOG NUMBER: 58-164

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Mouse TLR6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 722-753 amino acids from the C-terminal region of mouse TLR6.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
91 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Tlr6
Additional Names:
Toll-like receptor 6, CD286, Tlr6
Protein Accession Number:
Q9EPW9
Ncbi Gene Id Number:
21899
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TLR6, a Type I membrane protein that belongs to the Toll-like rceptor family, participates in the innate immune response to Gram-positive bacteria and fungi. It acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. The protein recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. It binds to TLR2 via their respective extracellular domains, and to MyD88 via their respective TIR domains. TLR6 is detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells.
Background Reference 1:
Ozinsky, A., et al., Proc. Natl. Acad. Sci. U.S.A. 97(25):13766-13771 (2000).
Background Reference 2:
Takeuchi, O., et al., Gene 231 (1-2), 59-65 (1999).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-164

List Size:
400 ul

List Price:

$495.00

Datasheet
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